A latent ability to persist: differentiation in Toxoplasma gondii

A critical factor in the transmission and pathogenesis of Toxoplasma gondii is the ability to convert from an acute disease-causing, proliferative stage (tachyzoite), to a chronic, dormant stage (bradyzoite). The conversion of the tachyzoite-containing parasitophorous vacuole membrane into the less permeable bradyzoite cyst wall allows the parasite to persist for years within the host to maximize transmissibility to both primary (felids) and secondary (virtually all other warm-blooded vertebrates) hosts. This review presents our current understanding of the latent stage, including the factors that are important in bradyzoite induction and maintenance. Also discussed are the recent studies that have begun to unravel the mechanisms behind stage switching

Distributional Survey of the Fishes of Ten Mile Creek in Southeastern Arkansas

A survey of the fishes of Ten Mile Creek was conducted during 1976 to 1979. The ichthyofauna of Ten Mile Creek is typical of lowland drainage systems in southeastern Arkansas. Fifty-three species representing 13 families and 23 genera were collected. Etheostoma parvipinne was locally abundant in the headwaters, and other vulnerable or rare species included Notropls maculatus, Fundulus notti, Fundulus chrysotus, Erimyzon sucetta, Moxostoma poecilurum, and Lepomis punctatus. Eight specimens of Notropis hubbsi were collected

Bromodomains in Protozoan Parasites: Evolution, Function, and Opportunities for Drug Development

Parasitic infections remain one of the most pressing global health concerns of our day, affecting billions of people and producing unsustainable economic burdens. The rise of drug-resistant parasites has created an urgent need to study their biology in hopes of uncovering new potential drug targets. It has been established that disrupting gene expression by interfering with lysine acetylation is detrimental to survival of apicomplexan (Toxoplasma gondii and Plasmodium spp.) and kinetoplastid (Leishmania spp. and Trypanosoma spp.) parasites. As "readers" of lysine acetylation, bromodomain proteins have emerged as key gene expression regulators and a promising new class of drug target. Here we review recent studies that demonstrate the essential roles played by bromodomain-containing proteins in parasite viability, invasion, and stage switching and present work showing the efficacy of bromodomain inhibitors as novel antiparasitic agents. In addition, we performed a phylogenetic analysis of bromodomain proteins in representative pathogens, some of which possess unique features that may be specific to parasite processes and useful in future drug development

The effects of stellar winds on the magnetospheres and potential habitability of exoplanets

Context: The principle definition of habitability for exoplanets is whether they can sustain liquid water on their surfaces, i.e. that they orbit within the habitable zone. However, the planet's magnetosphere should also be considered, since without it, an exoplanet's atmosphere may be eroded away by stellar winds. Aims: The aim of this paper is to investigate magnetospheric protection of a planet from the effects of stellar winds from solar-mass stars. Methods: We study hypothetical Earth-like exoplanets orbiting in the host star's habitable zone for a sample of 124 solar-mass stars. These are targets that have been observed by the Bcool collaboration. Using two wind models, we calculate the magnetospheric extent of each exoplanet. These wind models are computationally inexpensive and allow the community to quickly estimate the magnetospheric size of magnetised Earth-analogues orbiting cool stars. Results: Most of the simulated planets in our sample can maintain a magnetosphere of ~5 Earth radii or larger. This suggests that magnetised Earth analogues in the habitable zones of solar analogues are able to protect their atmospheres and is in contrast to planets around young active M dwarfs. In general, we find that Earth-analogues around solar-type stars, of age 1.5 Gyr or older, can maintain at least a Paleoarchean Earth sized magnetosphere. Our results indicate that planets around 0.6 - 0.8 solar-mass stars on the low activity side of the Vaughan-Preston gap are the optimum observing targets for habitable Earth analogues.Comment: 8 pages, 3 figures, accepted to Astronomy and Astrophysic

Habitat and bycatch effects on population parameters of inshore lizardfish (Synodus foetens) in the north central Gulf of Mexico

We examined the effect of habitat and shrimp trawl bycatch on the density, size, growth, and mortality of inshore lizardfish (Synodus foetens), a nonexploited species that is among the most widespread and abundant benthic fishes in the north central Gulf of Mexico. Results of quarterly trawl sampling conducted from spring 2004 through spring 2005 revealed that inshore lizardfish are most abundant on sand habitat, but larger fish are more common on shell rubble habitat. There was no significant difference in fish density between habitats exposed to shrimp trawling on the open shelf versus those habitats within a permitted artificial reef zone that served as a de facto no-trawl area; this finding indicates that either inshore lizardfish experienced minimal effects from trawling or, more likely, that fish moved between trawled and nontrawled habitats. Exploitation ratio (bycatch mortality/total morality) estimates derived from catch curve analysis ranged from 0.43 inside the artificial reef zone to 0.55 outside the reef zone, thus indicating that inshore lizardfish are subject to significant fishing mortality in the north central Gulf of Mexico despite the lack of a directed fishery for the species. We infer from this result that effects of shrimp trawl bycatch may be significant at the population level for nonexploited species and that a broader ecosystem-scale examination of bycatch effects is warranted

The solar wind in time – II. 3D stellar wind structure and radio emission

In this work, we simulate the evolution of the solar wind along its main-sequence lifetime and compute its thermal radio emission. To study the evolution of the solar wind, we use a sample of solar mass stars at different ages. All these stars have observationally reconstructed magnetic maps, which are incorporated in our 3D magnetohydrodynamic simulations of their winds. We show that angular-momentum loss and mass-loss rates decrease steadily on evolutionary time-scales, although they can vary in a magnetic cycle time-scale. Stellar winds are known to emit radiation in the form of thermal bremsstrahlung in the radio spectrum. To calculate the expected radio fluxes from these winds, we solve the radiative transfer equation numerically from first principles. We compute continuum spectra across the frequency range 100 MHz to 100 GHz and find maximum radio flux densities ranging from 0.05 to 2.2 μJy. At a frequency of 1 GHz and a normalized distance of d = 10 pc, the radio flux density follows 0.24 (Ω/Ω☉)0.9 (d/[10pc])-2μJy, where Ω is the rotation rate. This means that the best candidates for stellar wind observations in the radio regime are faster rotators within distances of 10 pc, such as κ1 Ceti (0.73 μJy) and χ1 Ori (2.2 μJy). These flux predictions provide a guide to observing solar-type stars across the frequency range 0.1-100 GHz in the future using the next generation of radio telescopes, such as ngVLA and Square Kilometre Array

Erratum: The solar wind in time II: 3D stellar wind structure and radio emission

This is an erratum to the paper ‘The solar wind in time - II: 3D stellar wind structure and radio emission’, which was published in MNRAS, 483(1), 873, 2019 (Ó Fionnagáin et al. 2019)

Evaluating the GCN5b bromodomain as a novel therapeutic target against the parasite Toxoplasma gondii

Toxoplasma gondii is a protozoan parasite of great importance in human and veterinary health. The frontline treatment of antifolates suffers a variety of drawbacks, including toxicity and allergic reactions, underscoring the need to identify novel drug targets for new therapeutics to be developed. We previously showed that the Toxoplasma lysine acetyltransferase (KAT) GCN5b is an essential chromatin remodeling enzyme in the parasite linked to the regulation of gene expression. We have previously established that the KAT domain is a liability that can be targeted in the parasite by compounds like garcinol; here, we investigate the potential of the bromodomain as a targetable element of GCN5b. Bromodomains bind acetylated lysine residues on histones, which helps stabilize the KAT complex at gene promoters. Using an inducible dominant-negative strategy, we found that the GCN5b bromodomain is critical for Toxoplasma viability. We also found that the GCN5-family bromodomain inhibitor, L-Moses, interferes with the ability of the GCN5b bromodomain to associate with acetylated histone residues using an in vitro binding assay. Moreover, L-Moses displays potent activity against Toxoplasma tachyzoites in vitro, which can be overcome if parasites are engineered to over-express GCN5b. Collectively, our data support the GCN5b bromodomain as an attractive target for the development of new therapeutics